recombinant mouse tgf-beta 1 protein  (Bio-Techne corporation)

Journal: Biomolecules

Article Title: Glycogen Hydrogel Loaded with Schistosoma japonicas Peptide SJMHE1 Improves Skin Wound Healing

doi: 10.3390/biom16030392

Figure Lengend Snippet: Macrophages treated with SJMHE1 promote fibroblast and HUVEC migration through TGF-β1/Smad3 pathway and VEGFA expression. ( A ) TGF-β1 protein expression in RAW264.7 cells assessed by Western blotting. ( B ) p-Smad3 protein expression in L929cells assessed by Western blotting. ( C ) Relative mRNA expression of COL1a1 in L929 cells. ( D ) Cell scratch assay and number of migrating L929 cells (scale bar = 200 μm). ( E ) p-Smad3 protein expression in HUVECs assessed by Western blotting. ( F ) Cell scratch assay and migration of HUVECs (scale bar = 200 μm). ( G ) VEGFA expression in THP-1-induced macrophages. ( H ) Representative images of vascular network formation and tube length quantification in HUVECs in vitro (scale bar = 200 μm). Data are presented as mean ± SD ( n = 3). ns = no significance, * p < 0.05, ** p < 0.01, and *** p < 0.001. Original Western blot images are provided in the .

Article Snippet: After washing with PBS, L929 cells and HUVECs were treated with the following low-serum (2% FBS) conditioned media: CM-Control, CM-SJMHE1, CM-SJMHE1 supplemented with 5 μg/mL TGF-β1 neutralising antibody (BE0057, Bio X Cell, Lebanon, PA, USA), or normal medium supplemented with 10 ng/mL TGF-β1 recombinant protein (HY- P70648 , MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Migration, Expressing, Western Blot, Wound Healing Assay, In Vitro

Journal: Biomolecules

Article Title: Glycogen Hydrogel Loaded with Schistosoma japonicas Peptide SJMHE1 Improves Skin Wound Healing

doi: 10.3390/biom16030392

Figure Lengend Snippet: SJMHE1-gel treatment increases TGF-β1, p-Smad3, and VEGFA expression in mice. ( A ) Representative immunohistochemical staining images of TGF-β1, p-Smad3, and VEGFA on day 7 (scale bar = 50 μm). ( B ) Quantification of TGF-β1, p-Smad3 ( C ) and VEGFA ( D ) expression. Data are presented as mean ± SD ( n = 6). ns = no significance, * p < 0.05, ** p < 0.01.

Article Snippet: After washing with PBS, L929 cells and HUVECs were treated with the following low-serum (2% FBS) conditioned media: CM-Control, CM-SJMHE1, CM-SJMHE1 supplemented with 5 μg/mL TGF-β1 neutralising antibody (BE0057, Bio X Cell, Lebanon, PA, USA), or normal medium supplemented with 10 ng/mL TGF-β1 recombinant protein (HY- P70648 , MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Expressing, Immunohistochemical staining, Staining